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Louis Pasteur's Germ Theory Experiments

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Louis Pasteur's germ theory experiments were pivotal in disproving the concept of spontaneous generation and establishing the germ theory as a cornerstone of biology. His most famous experiment involved using a swan-necked flask to demonstrate that microorganisms in the air, rather than the broth itself, caused contamination. By boiling broth in a flask with a curved neck, Pasteur prevented airborne particles from reaching the broth, showing it remained sterile unless exposed to external contaminants[1][3][5]. Pasteur's work extended beyond this experiment, linking microorganisms to fermentation and disease. He discovered that wine soured due to microbial contamination and developed pasteurization to kill pathogens in liquids[2][3]. His findings revolutionized medicine, food safety, and microbiology, laying the groundwork for modern germ theory and the understanding that infectious diseases are caused by microorganisms[3][4].